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Twist for Element, Trinity Freestyle™ Fast Hyb Product Sheet
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One Enrichment Workflow. Many Possibilities.
The Twist for Element, Trinity Freestyle Fast Hyb kits provide a user-friendly solution for the enrichment of NGS libraries containing P5 and P7 adapter sequences for sequencing on Element Biosciences platforms.
The traditional hybridization process for targeted panels, like whole exome panels, involves a time-consuming process with multiple temperature-controlled washes, PCR-based amplification, and library quality control.
With the simplified Trinity workflow, you’ll hybridize your DNA library to the target probes of interest, incubate with the panel probes for as little as one hour with our fast hyb reagents, and load it directly on your AVITI sequencer. This drastically reduces manual steps and additional amplification resulting in higher library complexity and lower duplication rates.
KEY BENEFITS
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Streamlined Workflow, Less Hands-on Time
- Go from sample to sequencer in as few as five hours
- On-flow cell enrichment removes binding, wash, amplification, and pooling steps
- Rapid, 1-hour hybridization time eliminates overnight waits
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Flexibility Without Compromise
- Seamlessly compatible with any Twist sequencing library using P5 and P7 adapters
- Preserves AVITI's exceptional data quality across applications
- Dual, independent flow cells deliver adaptable throughput
For research use only. Not for use in any diagnostic or clinical procedures.
Want to Learn More?
Request additional information about how to streamline your sequencing workflows on Element Bio’s AVITI system.
Rapid Exome Sequencing Using Twist Workflow Kits Reduces Hands-On Time
Comparison of the estimated workflow times for Standard Hybridization v2 with a 16-hour overnight hybridization and the Trinity Freestyle Fast Hyb workflow with a 1-hour hybridization time. Each workflow produces exome-enriched libraries compatible with AVITI sequencing.
Whole Exome and Human Genotyping Panels Have High Sequencing Performance on AVITI
Performance comparison of the native Trinity Fast Hyb workflow and the new Trinity Freestyle workflow paired with Twist Exome 2.0 plus Comprehensive Spike-in. There were 24 exomes sequenced on an AVITI and downsampled to 25 million reads. We also demonstrate the performance of Twist’s FlexPrep UHT paired with Twist Genotyping Human 600k panel for high-throughput genotyping of human samples. There were 120 samples sequenced on an AVITI24, reaching 13 to 28 million reads per sample. Values shown are averages ± standard deviation based on Picard metrics..
PRODUCT SKUS
127554
Twist Trinity Freestyle Fast Hybridization Kit, 96 Samples (Includes materials for 12 Hyb reactions and Trinity Freestyle binding reagent)
126889
Twist Adapter System - Trinity Freestyle, 96 Samples Plate 1, (Includes UDI primers for Trinity Freestyle and Universal Adapters)
126776
Trinity Freestyle Fast Hyb Binding Reagent, 12 Hyb Reactions
109298
Twist Alliance Long-Read PGx Panel, 2 Reaction Kit
106750
Twist Fast Hybridization Reagents, Element Trinity Compatible, 12 Reactions
126855
Twist Exome 2.0 + Comp. Spike-in, Trinity FS, 96 Samples
*All AVITI Compatible reagents available for individual purchase.
For research use only, use of Twist’s website and products are subject to Twist’s Terms and Conditions.
Twist Bioscience HQ
681 Gateway Blvd
South San Francisco, CA 94080
