The Twist cfDNA Library Preparation with TrueAmp Polymerase Mix Kit prepares high quality libraries from circulating cell free DNA.
The Twist cfDNA Library Preparation Kit provides our highest quality library preparation solution, offering not only robust performance with low sample input, but also high conversion rates.
Engineered Performance for Reliable, Scalable cfDNA Workflows
The cfDNA workflow leverages engineered GC balance and high fidelity amplification. This design delivers high amplification yield while minimizing PCR introduced errors that can compromise low frequency variant detection.
The result is uniform, high-quality libraries in a fast, 2-hour workflow. Aptamer-based hot-start control provides room-temperature stability for automated setup, while the single-tube 2× mastermix streamlines both WGS and Target Enrichment workflows.
*For research use only. Not for use in any diagnostic or clinical procedures
Twist TrueAmp Polymerase Mix
Low bias, high fidelity amplification engineered for NGS Workflows
Customer Testimonial
"We have been conducting genomic analysis of genetic mutations that accumulate in solid organs and are constructing a detection system that can reliably detect low-frequency variants of somatic mutations. By using the Twist cfDNA Library preparation kit, we not only obtain a library yield comparable to the conventional method from a small amount (1.5 ng) of fragmented DNA even if we reduce one less PCR cycle, but also increase the average coverage of the existing target capture sequence by 30% after constructing error-corrected reads using unique molecular indices. Twist's ligation process is simple and quick for library preparation and is expected to expand the application of molecular barcoding in trace systems." - Dr. Nobuyuki Kakiuchi
Associate Professor, The Hakubi Project, Department of Pathology and Tumor Biology, Graduate School of Medicine, Kyoto University, Japan
*Results are specific to the institution where they were obtained and may not reflect the results achievable at other institutions.
High Fidelity Amplification with PCR
High Fidelity Amplification with PCR Free–Like Error Rates cfDNA libraries prepared with Twist TrueAmp Polymerase show substitution error rates that are effectively equivalent to PCR-free cfDNA libraries across all measured base changes. The amplified workflow maintains the same low error profile observed in PCR-free preparation.
Figure 1: Comparable Base Substitution Error Rates with and without TrueAmp Amplification. The Twist cfDNA Library Preparation Kit was used to generate libraries with Twist cfDNA Pan-Cancer Reference Standard v2 as the input material. Twist Full Length Unique Dual Index (UDI) Adapters were used during ligation for the PCR-free condition and Twist Universal Adapters were used during ligation for the PCR condition. The PCR condition was amplified using 6 cycles with Twist TrueAmp Polymerase mix and UDI primers. The libraries were pooled, sequenced and analyzed. Comparable error rates are seen between PCR-free and TrueAmp libraries.
Higher Conversion Rate = Higher Yield
Following library generation, samples were eluted in 20 μL of water and 1 µL was quantified with QubitTM dsDNA Broad Range Kit.
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Higher Sensitivity & Higher Accuracy
Libraries were single-plex captured with a custom 50kb oncology panel targeting 217 SNP variant sites in the Pan-Cancer standard material. Variants Missed is calculated by summing sites with less than 1 variant family divided by total detectable variant sites.
Improved Duplexing = Higher Complexity
Libraries were single-plex captured with a custom 50kb oncology panel targeting variant sites in the cfDNA standard material with present or updated TE recommendation.
Low Input Proven with native cfDNA
Following library generation, samples were eluted in 20 μL of water and 1 μL was quantified with QubitTM dsDNA Broad Range Kit.
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