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Complete coverage, expanded discovery

Get the full picture with Whole Genome Sequencing.

Whole Genome Sequencing (WGS) analyzes an organism’s entire genome—both coding and non-coding regions—offering a complete view beyond just exome sequencing. By capturing variants in regulatory regions, introns, structural elements, and mitochondrial DNA, WGS enables insights into complex diseases, rare conditions, and traits not explained by coding sequences alone. As sequencing costs decline and analysis tools improve, WGS is becoming a powerful tool in both clinical diagnostics and genomic research.

Whole Genome
Sequencing
BENEFITS
  • Covers billions of bases for the complete genome
  • Offers comprehensive coverage for broader examination of genome alterations, including novel variants in both coding and noncoding regions
  • Readily enables retrospective analysis as new genetic biomarkers emerge
  • Key applications include rare disease research, population genomics, and functional genomics
Whole Genome Sequencing Diagram
Whole Exome
Sequencing
BENEFITS
  • Targets only 1-2% of the entire genome, focusing on protein coding regions and regions containing known disease causing variants
  • Useful for routine variant determination
  • Enables greater depth of sequencing for detection of target variants with improved sensitivity
  • Reduces sequencing reads required substantially, enables greater throughput of samples per sequencing run
Whole Exome Sequencing Diagram
How it works

Whole Genome Sequencing Workflow

WGS requires the preparation of DNA libraries that go directly onto a sequencer - no target enrichment step is required. There are a myriad of ways NGS libraries can be prepared that optimize for different research goals: sample or analyte type, workflow efficiency, coverage requirements, etc. Twist offers a comprehensive suite of library preparation kits and accessory products, empowering you to tailor your WGS workflows to your unique needs.

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Why choose us

Streamline WGS with Precision-Engineered
Library Prep

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Tailored Enzymes to Improve Your Workflow

Twist’s library preparation kits incorporate proprietary enzymes designed using advanced AI/ML modeling and our high-throughput DNA synthesis platform. This unique enzyme development pipeline ensures that each enzyme is custom-engineered to maximize performance in NGS workflows - ensuring you receive superior results.
flexibility
Superior Performance for High-Quality Data

Get more insights from your data. Twist’s high-yield, high-complexity libraries provide exceptional sequencing depth, reduce duplicates, and improve genome coverage efficiency - even for difficult regions like GC-rich areas.
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Flexible Workflows for Any Application

With a range of library preparation kits tailored for diverse research goals and accessories such as adapters to meet your unique needs, there’s a Twist kit to suit any application. Plus, our team of skilled field application scientists (FAS) is always ready to help optimize your workflow and ensure you get the data you need.
What we offer

Featured WGS solutions

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Enzymatic Fragmentation 2.0 Library Prep Kit

Workhorse kit for most applications.

 

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cfDNA Library Prep Kit

Highest sensitivity kit optimized for low-input and cfDNA.

 

 

 

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FlexPrep UHT Library Prep

Highest throughput option with a streamlined workflow.

 

 

Fragmentation Process
Enzymatic
Mechanical
Enzymatic
Throughput
Middle
Lowest
Highest
Input Range
N/A
≤1 ng
30–300 ng
Best For
Clinical
Liquid Biopsy
High throughput studies e.g.
Population Genomics and
Agrigenomics
Special Features
N/A
Highest library conversion
rate
  • Self-normalizing DNA input
  • Integrated indexing for high-capacity multiplexing
  • Automation-friendly
What we offer

Explore the rest of the WGS lineup

Mechanical Fragmentation Library Preparation Kit
Library Prep

Mechanical DNA fragmentation for robust sequencing.

Twist Adapter Systems
Adapters

Optimized for mouse model genetics and disease studies.

Optimize your WGS workflow

Suggested accessories

population
Highlighted applications

Population Genomics

WGS enables comprehensive analysis of genetic variation across human populations, supporting studies in diversity, adaptation, ancestry, and disease association. Twist’s high-throughput WGS solutions are ideal for large-cohort research, enabling precise variant detection and uniform coverage critical for trait mapping and population-scale insights.

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Twist products are for research use only. The products presented here are not intended for the diagnosis, prevention, or treatment of a disease or condition. Twist Bioscience assumes no liability regarding use of the product for applications in which it is not intended. The results are specific to the institution to which they were obtained. The results presented are customer-specific and should not be interpreted as indicative of performance across all institutions.

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Twist Bioscience HQ
681 Gateway Blvd
South San Francisco, CA 94080