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Introducing Gene Pools

Gene Pools. Build something brilliant with unprecedented access to unlimited DNA.

Gene Pools are a revolution in pooled DNA synthesis. Our proprietary pooled assembly platform assembles pools of oligos into pools of genes ranging from 300bp to 1.8 kb. Our Gene Pools platform completely solves the challenges common in pooled assembly workflows, offering an up to 1:3000 error rate*, a 0% chimera rate**, 90% sequence representation within 10x the mean***, and <1% dropout rates**. Cut through the noise caused by unwanted or overrepresented sequences and leave no sequence unscreened

 

BENEFITS

Customizable design options

  • Our pooled gene assembly platform has no limitations on homology in sequence design, build exactly the sequences you desire in a single pool.
  • Lengths from 300 bp to 1.8 kb are available, giving ample space for sequence designs.

Unique-to-industry chimera-free pooled gene assembly

  • Industry leading low error rates of up to 1:3000 *
  • 0% chimeras, less than 1% dropout rate **
  • Uniform pools, with a 95th/5th percentile of ≤ 10***

Rapid synthesis and assembly

  • Synthesis and shipment in as few as 10 Business Days****

How Gene Pools are changing high throughout screening

Read this article from Emily Leproust and hear how Gene Pools were developed. Learn how our first-to-market 0% chimera rate pooled assembly technology marks a paradigm shift in high throughput screening.

Read the article

* Error rate for the previous year was an average of 1/2911 with a standard deviation of 422.

** Dropout rate refers specifically to accepted sequences. Of fragments accepted in fiscal year 2025, >99% were shipped.

*** Uniformity metrics are only applicable for sequences within a maximum 15% length variation.

**** Gene pools up 50k fragments are shipped within 10BD, up to 150k fragments are shipped within 15BD. This timeframe refers to the typical processing and handling time within our facilities before your order is handed over to the shipping carrier. Actual delivery times will vary depending on your location, the chosen shipping method, and the carrier's handling procedures.

Gene Pools

To rigorously benchmark this platform, we assembled multiple gene pools, each consisting of thousands of sequences at lengths between 650bp and 1.8kb. These pools were designed with mean pairwise similarities up to 97%, classifying them as “chimera-prone” if they were built through standard pooled assembly methods. We then employed high-fidelity PacBio single-molecule sequencing to precisely quantify the percentage of perfect molecules (no synthesis errors, template switching, or chimeras). 

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Let's Get Started

 

  • Step 1: Complete the contact details form on this page
  • Step 2: Download the MGF Submission Form and fill out all fields in the form for your MGF design
  • Step 3: Upload the completed submission form to the "Submit File" tab on this page
     

If you have any questions, please feel free to email us at customersupport@twistbioscience.com

Here is your file!

MGF_SubmissionForm.xlsx

 

 

Let's Get Started

 

  • Step 1: Complete the contact details form on this page
  • Step 2: Download the MGF Submission Form and fill out all fields in the form for your MGF design
  • Step 3: Upload the completed submission form to the "Submit File" tab on this page
     

If you have any questions, please feel free to email us at customersupport@twistbioscience.com

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