In as fast as four days*, we ship 100% accurate cloned genes prepped to your specification, ready to be dropped into your downstream workflow.
Get reliable yields at every prep scale, backed by fluorometric quantification.
Percent orders meeting minimum yield. Shipped constructs between April 2025 - September 2025.
Keep projects moving with predictable timelines on every order, regardless of scale, complexity or sequence length.
Order to shipment, Micro scale, April through September 2025. Express Service turnaround time 4 – 7 business days, Standard Service 10 business days.
Every DNA order is routine and reliable, with 98.4% manufacturing success on low and medium complexity sequences. Genes only leave the factory when NGS verified as a 100% match to your desired sequence.
Percentage of accepted sequences that pass NGS verification on initial synthesis, by complexity score. Data recorded from between April 2025 to September 2025. Sequences that don’t pass enter the re-synthesis pipeline at no additional cost.
Price estimator
Estimate†
Price
$1,700.00 – $2,550.00
Turnaround time (business days)
4 – 10 (Low/Medium complexity)
10 – 15 (High complexity)
Estimates shown include variations in sequence complexity and speed of service (Standard or Express).
Get a more accurate estimate
Input your sequences to check their manufacturability and compare pricing options.
Vectors
Can’t find a vector in our catalog? Onboard your vector once. Use it forever.
65+ vectors available in our vector catalog
Available in your Twist account for immediate use.
Onboard your own vectors seamlessly online
Your vectors will be available on your Twist account to use on every future order. Learn more about vector onboarding.
Your IP stays yours
Your vector is tied to you. We store it and only use it for your requests.
Share your vectors with collaborators
On your request, our support team can make your vectors available to other Twist User accounts at no extra cost.
Product specifications
| Specification | Value | Details |
|---|---|---|
| Product format | Circular dsDNA | |
| Length | 0.3 kb – 7.0 kb | |
| Complexity | Low | Medium | High |
A sequence’s complexity score is based on the combined presence, frequency, and length of various features within the design of a sequence. Impacts the ease and speed of production. High complexity sequences are produced though a series of specialized manufacturing workflows. Supported features include:
|
| Codon optimization | 100s of target organisms | Large language model-driven for protein expression. |
| Specification | Value | Details |
|---|---|---|
| Express service (Low/Medium complexity) Add-on | 4 – 7 business days* | At default scale (Micro). Larger scale preps may increase turnaround time. |
| Standard service (Low/Medium complexity) | 10 business days* | At default scale (Micro). Larger scale preps may increase turnaround time. |
| Standard service (High complexity) | 15 business days* | At default scale (Micro). Larger scale preps may increase turnaround time. |
| Vectors |
|
Includes cloning, expression, and IVT vectors. View our catalog One-time onboarding for new custom vectors with unlimited use thereafter. |
| Custom vector onboarding Add-on | 10 business days | Learn about custom vector onboarding |
| Synthesis platform | Silicon chip (proprietary) | |
| Synthesis chemistry | Phosphoramidite (proprietary) | |
| Carbon footprint | ~18 kg CO2e per gene | Reagent and energy consumption are 0.16% that of contemporary gene synthesis methods, due to our use of miniaturized silicon chip chemistry. |
| Animal-origin-free manufacturing Add-on | AOF, BSE/TSE | Animal origin free certification is included with your order. |
| Specification | Value | Details |
|---|---|---|
| Prep scalesAdd-on |
Default:
Add ons:
|
Midi and Maxi scale: +3 business days |
| Target yieldAdd-on | Minimum or exact yield | Available at Mini scale. |
| Purity gradeAdd-on |
|
|
| Concentration normalizationAdd-on | 0.25 – 1.0 mg/mL | Available at Midi and Maxi scale. |
| Glycerol stockAdd-on | Available for all orders | |
| Delivery formatAdd-on | Dried down or resuspended |
|
| Container |
|
Micronic tubes and deep well plates available at Midi and Maxi scales. |
| Shipment conditions | Ambient or frozen on dry ice |
|
Every Clonal Gene we ship includes a Certificate of Analysis (COA) that contains the following information.
| Specification | Value | Details |
|---|---|---|
| Yield measurement | Fluorometric quantification | |
| Sequence identity | 100% match | |
| Sequencing method | NGS (long and/or short read) | |
| Poly-A analysisAdd-on | NGS |
Customers often pair Twist Genes with the following products
Partner with our team of experts to navigate the entire discovery funnel from target to lead.
High-diversity, silicon-synthesized oligo pools designed for high-throughput screening and complex library construction.
Explore vast sequence space with high-diversity libraries where every variant is base-by-base synthesized and NGS-verified to match your design.
Twist’s Technical Support team is available to help you tackle your biggest research challenges. If you have any questions about your project, our scientists are here to find the answer.
For Research Use Only.
Not for Diagnostic Procedures.
*This timeframe refers to the typical processing and handling time within our facilities before your order is handed over the shipping carrier. Gene Fragments are shipped in 2-4 business days. Eligible Express Genes ship in 4-7 business days. Average turnaround time for Clonal Genes is 10 business days. Our target completion timeline for clonal genes scored as complex is 15 business days. Turnaround time for Clonal Genes is subject to change based on customizations and sequence complexity. New vector onboarding for Clonal Genes will add additional turnaround time. Orders placed outside of the US will incur additional delivery time.
†This is for illustrative purposes and the stated benefits, support, benefit calculations and other results are estimates only, based on input from a limited number of existing Twist customers and various assumptions made by the potential customers. This estimate does not constitute a proposal or quote.
Twist Bioscience HQ
681 Gateway Blvd
South San Francisco, CA 94080
Genes
NGS
Antibodies
IVDR Solutions
