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Introducing Twist Multiplexed Gene Fragments

Twist Multiplexed Gene Fragments are double stranded gene fragment sequences of up to 500 base pairs in length that are delivered as a pool instead of one fragment per well matrix. This pooled format of fragments enables high-throughput screening applications such as prime editing, ultra-complex CRISPR-based functional screening, peptide and protein engineering, Ab discovery, and massively parallel reporter assays (MPRA), where cost per fragment can be a barrier to the desired number of variants.
Multiplexed Gene Fragments are synthesized from user defined sequences in lengths of up to 500 base pairs per gene. These user-defined sequences are initially synthesized into oligo pools of up to 500 nucleotides in length and are then amplified in double-stranded DNA, and delivered as multiplexed gene fragments that have been carefully analyzed to ensure >90% of genes in the pool are the correct length. This product is optimized for rapid, high-throughput screening applications.

BENEFITS

Customizable

  • Large scale pooled fragment synthesis with unprecedented design options
  • Lengths up to 500 bp for high-throughput screening

INDUSTRY-LEADING, HIGH-QUALITY FRAGMENTS

Industry-leading, High-Quality Fragments

  • Maximized pool quality with low error rates
  • Even representation across fragment lengths and GC contents

FLEXIBILITY

Flexibility

  • Total control over the precise sequences in your pool
  • Expanded coding region

500nt Multiplexed Gene Fragments encode antibody sequences in AI-guided Discovery programs

Read how the Baker Lab used Multiplexed Gene Fragments and RFDiffusion to to discover novel antibody binders de novo.

Read application note

A Multiplexed Gene Fragment pool

Twist’s Multiplexed Gene Fragment pools achieve comprehensive representation of every sequence ordered, to ensure precise control over variant construction for more targeted and rational screening.
A Multiplexed Gene Fragment pool of dual gRNA variants of 500 bp shows complete fragment representation with minimal dropouts (Fig 1) as does a pool of triple gRNA variants of 450 bp (Fig 2). Lastly, a Multiplexed Gene Fragment pool with variants containing GC content across a wide range including fragments with up to 80% GC shows high representation and minimal bias (Fig 3).

Multiplexed Gene Fragment
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Figure 1. Graph of read count of each of the mapped dual gRNA variants (X-axis) for an amplified pool of Multiplexed Gene Fragments of 500 bp.

 

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Figure 2. Graph of read count of each of the mapped triple gRNA variants (X-axis) for an amplified pool of Multiplexed Gene Fragments of 450 bp.

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Figure 3. GC plots of an amplified pool of Multiplexed Gene Fragments of 500 bp length were created and show minimal bias among a wide range of GC content up until 80%. X-axis are categories of 14 groups of sequences with different GC content. Y-axis is the read count.

 

Product Specifications

YieldMinimum of 200 ng of amplified dsDNA
Turnaround time8-12 business days
Delivery FormatDried-down, dsDNA pooled in a 2 mL tube
Length301 - 500 bp
Pool sizeNo minimum, no maximum
Uniformity90% of sequences are within ~3x of the mean*
Quality Control (QC)Fragment analysis to ensure >90% of genes in a pool are the correct length*
Error Rate1:2000 nt (from the original pool synthesis, before amplification)

*Terms and Conditions: Standard turnaround time for Multiplexed Gene Fragments is 8-12 business days. This will vary based on sequence length and complexity. Multiplexed Gene Fragment length is up to 500 base pairs, with up to 460 bp allotted for the variable region with a minimum of 40 bp conserved for the primer region. Quality control tests ensure >90% of gene sequences within a pool will be user defined length.


For research use only. Not for Diagnostic Procedures.

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Let's Get Started

 

  • Step 1: Complete the contact details form on this page
  • Step 2: Download the MGF Submission Form and fill out all fields in the form for your MGF design
  • Step 3: Upload the completed submission form to the "Submit File" tab on this page
     

If you have any questions, please feel free to email us at customersupport@twistbioscience.com

Here is your file!

MGF_SubmissionForm.xlsx

 

 

Let's Get Started

 

  • Step 1: Complete the contact details form on this page
  • Step 2: Download the MGF Submission Form and fill out all fields in the form for your MGF design
  • Step 3: Upload the completed submission form to the "Submit File" tab on this page
     

If you have any questions, please feel free to email us at customersupport@twistbioscience.com

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Testimonials

“

Twist's Multiplexed Gene Fragments push the limits of what we can synthesize, while fitting within the limits of what we can sequence. We can now explore sequence space in a targeted, deterministic fashion to build a holistic understanding of the mechanisms of protein binding. We will hopefully leverage that data to make new medicines.

Pierce Ogden

Co-founder and CSO, Manifold Biosciences inc

“

We are trying to tailor make proteins for specific design problems. Twist's 450bp Multiplexed Gene Fragments are the ideal length for us to screen libraries of candidate proteins for ligand binding using yeast display.

Jeffrey Chang

Graduate Student, Nick Polizzi Laboratory, Harvard University and Dana Farber Cancer Center

“

Regulatory elements have been studied deeply with traditional 120 base pair libraries, but in reality many regulatory elements are much longer than that. Using ≥450 base pair Multiplexed Gene Fragments from Twist we are now able to systematically screen for regulatory elements more finely tuned to drive gene expression in our specific cell type.

Josh Tycko

Postdoctoral Researcher, Department of Neurobiology Harvard Medical School

Testimonials

 

 

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